A Study Exploring the Effects of Cell Disruption Techniques on Lipid Recovery in Co-cultivated Microalgae and Oleaginous Yeast

Co-cultivation and effective downstream processing of microalgae oleaginous yeast are potential strategies to enhance lipid yield. The findings this work suggest that each cultivable partner should be screened optimized for the best mutualistic co-cultures. In case, T. pullulans was found most culture with S. obliquus, C. sorokiniana, protothecoides along additional cell disruption techniques. Co-cultures C.protothecoides T.pullulans had highest relative yield (RLY) 68.71%, 59.32%, 55.33%, 53.19%, 47.3% sonication, microwave, osmotic shock, freeze-drying, autoclave, respectively. Sonication concluded as technique five out nine co-cultures showing significant enhancement. Even though techniques, co-culture Y. lipolytica S.obliquus (RLY 37.11%) produced lipids which significantly higher than their respective monocultures (S. obliquus RLY 23.96%, 4.96%) without any process. R. glutinis enhanced C.sorokiniana sonication 51.44%) shock 57.70%), FAME analysis reported percentage total nearly 100% in case sorokiniana high content saturated fatty acids (SFAs). presence biofuel precursors like palmitic acid, linoleic oleic heptadecanoic confirms suitability production.